A total of 296 strains of protease-producing bacteria were isolated and purified from medium-temperature Daqu produced by ZhangGong LaoJiu Wine Co.Ltd.After calculating the ratio of transparent ring diameter to colony diameter and measuring the protease activities, a strain of high-yield protease bacteria, called DW-7, was screened out with a protease activity of 99.54 U/mL.
Through morphological observation, 16S rDNA sequence analysis, and physiological Mains Suppression Capacitor and biochemical tests, the isolated bacteria DW-7 was determined to be Bacillus velezensis.In addition, whole-genome sequencing (WGS), using PacBio and the Illumina platform, was performed.Gene annotation was then conducted using the Clusters of Orthologous Groups (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG), Non-Redundant Protein Sequence Database (NR), and Gene Ontology (GO) databases.The results showed that the genome of DW-7 was 3,942,829 bp long with a GC content of Basketball - Accessories - Accessories 46.
45%.A total of 3,662 protein-encoding genes were predicted, with a total length of 3,402,822 bp.Additionally, 2,283; 2,796; and 2,127 genes were annotated in the COG, KEGG, and GO databases, respectively.A total of 196 high-yield protease genes were mainly enriched in the metabolism of alanine, aspartic acid, glutamate, glycine, serine, and threonine, as well as ABC transporter and transporter pathways.